Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific CellPro™ Tris Buffered Saline, 20X, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.0) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Medchemexpress LLC TAE Powder / TAE Buffer | 100 POUCHES
TAE (Tris-acetate-EDTA) buffer is widely used in biological experiments, especially in agarose gel electrophoresis of nucleic acid. Linear dsDNA migrates quickly in electrophoresis, and DNA fragments (> 13 kb) can be separated effectively. TAE buffer is also suitable for DNA gel extraction.
- Consists of Tris-acetate and EDTA-2Na.
- Each pouch can be diluted to 1 L of 1x TAE buffer.
- Concentration of Tris-acetate is 40 mM and EDTA-2Na is 1 mM when diluted to 1 L of 1x TAE buffer.
- Provides a convenient way to make TAE solution.
- Eliminates the need to weigh and mix individual components.
- pH of the 1x TAE buffer is 8.3 ± 0.1 @ 25°C.
Alkali Scientific CellPro™ PBS Buffer, 1X , pH 7.4, 4L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Bioworld PBS/Fish Gelatin (10X), 200 mL
PBS/Fish Gelatin (1x) Phosphate Buffered Saline/fish gelatin 0.5% blocks, diluents, and coating solutions.Technical Information 1x: Fish Gelatin 0.5%PBS (1x)pH range: 7.4 (+/- 0.1)
Rockland Immunochemicals 1.0 M Tris HCl pH 9.0 - MB-028-1000
Tris solution is an abbreviation of the organic compound known as tris(hydroxymethyl)aminomethane. Tris is extensively used in biochemistry and molecular biology. In biochemistry, Tris is widely used as a component of buffer solutions, such as in TAE and TBE buffer, especially for solutions of nucleic acids.
Growcells PBS 20X 2L EA
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-5000.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 5 Kilograms
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
![Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75070-100.0.JPG-250.jpg)
Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 100 Grams
HEPPSO (N-(2-Hydroxyethyl)piperazine-N'-(3-propanesulfonic acid)) is a zwitterionic buffering agent commonly used particularly in biochemical and molecular biology research. It is valued for its ability to maintain a stable pH in a variety of biological assays and experiments.
HEPPSO is often preferred for its buffering capacity over a wide pH range, typically between pH 6.8 to 8.2, making it suitable for various applications such as protein purification, enzyme assays, cell culture, and electrophoresis.
Additionally, HEPPSO is less likely to interfere with biological processes compared to some other buffering agents, making it a versatile choice for maintaining optimal conditions in biological experiments.
Zymo Research Corporation RNA Wash Buffer, 24 ml
RNA Wash Buffer (24 ml). For use with Zymo Research RNA purification kits. Sold individually or as part of a complete RNA purification kit.
Research Products International Corp PIPES DISODIUM SALT, 100G
pKa (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5.
Zymo Research Corporation RBC Lysis Buffer, 50 ml
RBC Lysis Buffer (50 ml). Sold individually or as part of a nucleic acid purification kit for the purificaiton of RNA.
![Research Products International Corp POPSO, Free Acid, [Piperazine-N-N'-bis(hydroxypropane sulfonic acid)], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P30400-25.0.JPG-250.jpg)
Research Products International Corp POPSO, Free Acid, [Piperazine-N-N'-bis(hydroxypropane sulfonic acid)], 25 Grams
CAS Number: 68189-43-5
Molecular Formula: C10H22N2O8S2
Molecular Weight: 362.4