Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Kd Medical Inc TRIS BUFFER, 2M, PH 8.0, 1L
Buffer used in a wide range of Molecular Biology applications. 2 Molar solution pH 8.0. One 1 Liter bottle.
Kd Medical Inc TRIS-HCL BUFFER 1M, PH 7.5, 1L
Used for molecular biology, protein chemistry, and biochemical applications. 1 Molar solution, pH 7.5. One 1 liter bottle.
Kd Medical Inc PBS 10X, PH 8.0
Standard buffer for many biomolecular procedures. One 1 Liter bottle.
Kd Medical Inc TAE 50X, 1L BOTTLE
TAE Buffer (Tris Actetate EDTA) is the most commonly used buffer for agarose DNA electrophoresis. 50X concentrated ready to be diluted to a 1X working solution. One 1 liter bottle.
Growcells PBS 20X 2L PK6
Sterile 20X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 160g, KCl 4g, Na2HPO4 28.8g, KH2PO4 4.8g
Growcells PBS 10X 1L CS12
Sterile 10X Phosphate-Buffered Saline solution concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: NaCl 80g, KCl 2g, Na2HPO4 14.4g, KH2PO4 2.4g
Kd Medical Inc TRIS-GLYCINE-SDS BUFFER 10X
Tris-Glycine-SDS PAGE Buffer (10X) is a concentrated solution of Tris-Glycine-SDS in distilled/deionized water, especially intended for use as the running buffer in SDS-PAGE of proteins. One 1 liter bottle.
Kd Medical Inc PBS BUFFER, 1X, PH 7.4, 500ML
1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. One 500ml bottle.
Kd Medical Inc TRIS BUFF HCL PH8.0 1L
Used for molecular biology, protein chemistry, and biochemical applications. 1 Molar solution, pH 8.0. One 1 liter bottle.
Kd Medical Inc PBS BUFFER,W/ CA+MG,1X, PH 7.4
1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. Solution contains Calcium and Magnesium. One 500ml bottle.
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml
Cellular crude extracts contain numerous endogenous enzymes such as phosphatases and proteases capable of degrading proteins in the extracts The most effective strategy to enhance the yield of intact proteins is through the addition of inhibitors targeting these known endogenous enzymes The Protease Inhibitor Cocktail is intended for the purification of His-tagged proteins to improve protein stability This cocktail functions by inhibiting proteases that degrade either phosphorylated or non-phosphorylated protein substrates This Protease Inhibitor Cocktail contains individual components including AEBSF Bestatin E-64 Phosphoramidon and Pepstatin A offering broad-spectrum specific inhibition against cysteine serine aspartic thermolysin-like proteases and aminopeptidases It has been optimized and validated specifically for use with His-tagged proteins The cocktail is dissolved in DMSO and supplied as a ready-to-use solution
GenDEPOT TBST(10X), pH 7.4 | 1L
TBST(10X) is a space-saving stock solution that is ideal for preparing TBS-Tween (TBS-T) wash buffers or blocking buffer diluent used for applications such as ELISA, Western blotting and other immunoassays. The 10X TBST (Tris-buffered saline with Tween™ 20 detergent) makes 25 mM Tris, 0.14M NaCl, 0.1% Tween-20, pH 7.4, when diluted to 1X with water.
Medchemexpress LLC Cetuximab PBS 5mg | 5mg
Cetuximab (PBS) is a human IgG1 monoclonal antibody that can inhibit EGFR The SPR method measured the Kd value of Cetuximab for EGFR to be 0 201 nM Cetuximab has a highly effective anti-tumor effect[1]
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
A measured mixture of chemically defined ingredients makes up a basal medium which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/f-12 is a completely defined synthetic medium which is protein-free.
Sigma Aldrich Fine Chemicals Biosciences HEPES solution 1 M pH 7.0
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.